Преклинические исследования

Система SARRP была создана для имитации условий клинической практики.

Имитация клинических протоколов лечения

Система SARRP была создана для имитации условий клинической практики. В системе имеется встроенная система конусно-лучевой КТ (КЛКТ) для таргетированного планирования при размере луча 0.5 мм.

Система может использоваться при проведении преклинических исследований с целью имитации клинических протоколов лечения, оценки возможных новых методов лечения, радиационных поражений, гистологии и биологических характеристик отдельных образцов.

Изучение рака по его локализации

Голова и шея:

These researchers attempted to use SARRP to mimic a clinical treatment. They used a head and neck human tumor in a nude mouse model. They imaged these mice on a PET and created a plan based on the area of interest and a boost to the area of high FDG uptake. This PET image was fused with SARRP CT image while the mouse was anesthetized. 10Gy was given using a 15mm collimator to the whole tumor. The boost was treated with 10Gy using a non-coplanar dynamic arc with the 5mm collimator. The researchers found that a PET/CT with the SARRP can allow for pre-clinical validation of PET image-guided dose escalation IMRT treatments.

Кости:

A study of the effects of radiation on a specific part of the bone. Mesenchymal stem cells survival was affected the addition of free radicals created during irradiation of the bone
GI tract:

In contrast to popular belief, there is not one central necrotic area but several very small repeating areas of hypoxia.

Поджелудочная железа:

This is a combination radiation plus radiosensitizer study in prostate cancer cell lines. AUY922, a Hsp90 inhibitor, caused radiosensitization in both prostate cancer cell lines used. Radiation Therapy plus AUY922 produced tumor growth delay compared to radiation alone or AUY922 alone.

Простата:

These researchers created Gold-Loaded brachytherapy spacers (GBS). These were implanted in prostate mice models and evaluated with SARRP CBCT over time to evaluate the release of the GBS diffusion. The burst and diffusion was also simulated by injecting into the tumor volume a solution of 20 mg Au/mL concentration into 20 uL of phosphate buffered saline. CBCTs were taken at 0.5, 48, 120, 144 and 160 hours after diffusion. The pixels were measured by their intensity. The GBS had a CT intensity that decreased 160% over the time period. The Au solution decreased in CT intensity by 140%. The researchers conclude that these two methods offer a lasting volume in the prostate which could allow for boost treatments and salvage brachytherapy.

Легкие:

Proteasome inhibitors were studied as a radiosensitizer with radiation in NSCLC tumors. They found decreases in the homologous recombination after proteasome inhibitors plus radiation causing double strand DNA breaks. They were also found to block the KF-κB pathway by interfering with degredation of IκBα. This rescued the bortezomib-induced radiosensitivity. Alternatively radiosensitivity could be caused by the induction of apoptosis. Many studies have shown an increase in apoptosis after bortezomib treatment. Reduced growth was seen in xenografts after a combination therapy of proteasome inhibitors and RT.

Череп:

In this study, Pulsed low dose rate irradiation is defined as 0.2Gy delivered 10 times separated by 3 minutes for each irradiation to complete a fraction. This was compared to Stereotactic Radiotherapy in 2Gy fractions. Both types of irradiation were given in 10 fractions. It was found that PLDR offered better tumor control and lower normal tissue sparing most likely due to preservation of the vascular density.

Для получения более подробной информации о системе SARRP и ее использовании для исследований опухолей, локализованных в упомянутых выше зонах, свяжитесь с нашей технической командой.

Неонкологические заболевания

Радиотерапия может использоваться для лечения доброкачественных заболеваний, либо для супрессии костного мозга перед трансплантацией. При проведении исследований радиация также может быть использована для нанесения повреждений с целью изучения побочных эффектов, доз и вариантов лечения.

Hedgehog pathway has been implicated in tumor recurrence. Inhibitors of this pathway have shown radioresistance (hepatocellular) and radiosensitivity (esophageal) in vitro. This study on non-small cell lung cancer showed no significant radioresistance in vitro. In vivo (xenograft and orthotopic models) both showed great radiosensitivity verified by CBCT on SARRP.

Для получения более подробной информации о системе SARRP и ее использовании для исследования опухолей, локализованных в упомянутых выше зонах, свяжитесь с нашей технической командой.

Золотые наночастицы

Радиосенсибилизаторы – это вещества, которые делают опухоль более восприимчивой к влиянию радиоактивного излучения. В тех случаях, когда радиотерапия входит в стандартный протокол лечения, но не имеет должного эффекта, радиосенсибилизаторы могут значительно повысить эффективность радиотерапевтического лечения.

Золотые наночастицы являются действенным инструментов в радиотерапии. Они могут использоваться с целью сделать ткань более восприимчивой к радиотерапии, помочь с определением местоположения опухоли и доставкой лекарственных средств.

These researchers created Gold-Loaded brachytherapy spacers (GBS). These were implanted in prostate mice models and evaluated with SARRP CBCT over time to evaluate the release of the GBS diffusion. The burst and diffusion was also simulated by injecting into the tumor volume a solution of 20 mg Au/mL concentration into 20 uL of phosphate buffered saline. CBCTs were taken at 0.5, 48, 120, 144 and 160 hours after diffusion. The pixels were measured by their intensity. The GBS had a CT intensity that decreased 160% over the time period. The Au solution decreased in CT intensity by 140%. The researchers conclude that these two methods offer a lasting volume in the prostate which could allow for boost treatments and salvage brachytherapy.

Система SARRP позволяет проводить визуализацию и облучение при помощи золотых наночастиц, позволяя исследователям изучить данные методы.

Иммунотерапия

Иммунотерапия имеет большое значение в области радиоонкологии и применяется в составе комбинированной терапии.
Принимая во внимание многообразие механизмов возникновения опухолей, данные комбинированные методы могут демонстрировать положительные результаты во время преклинических исследований.

Transforming growth factor-β (TGFβ) has pro-tumorigenic activities and immunosuppressive qualities. Currently there are TGFβ inhibitors in clinical trials. Radiation Therapy induces TGFβ activity which elicits immunogenic signals but creates immunosuppressive effects in the tumor microenvironment.

This group of researchers tested the inhibition of TGFβ in a breast cancer model with radiation. The mice were injected with TGFβ neutralizing antibody 1D11 on day 13 after implantation. They were irradiated 24 hours later with 5 fractions of 6Gy each. The TGFβ neutralizing antibody had no effect on the turmo growth. Radiation therapy plus TGFβ inhibition showed significant tumor regression compared to Radiation therapy alone. There was a large reduction in tumor-associated macrophages (CD11b+Gr1+/intF4/80+). They found that IL12 was upregulated and IL10 and CXCL1 were decreased.

The researchers concluded that TGFβ inhibitors sensitize tumor cells to radiation therapy and create a tumor microenvironment that promotes tumor control. TGFβ blockade sensitizes tumor cells to RT and generates a tumor microenvironment that promotes tumor control. TGFβ inhibition also alleviates tumor suppressive consequences of radiation and creates a pro-immunogenic signal. Together, these actions can achieve persistent tumor control.

Узнайте больше о системе SARRP.

Для получения более подробной информации о системе SARRP и ее использовании для исследования опухолей, локализованных в упомянутых выше зонах, свяжитесь с нашей технической командой.

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