Irradiation cabinets have been traditionally used for both in vitro and in vivo treatments. The SARRP offers the user an advantage with in vivo treatments by mimicking the geometry and movements of Human Radiation Therapy treatment. Research usually begins with an in vitro component. This allows the user to be able to visualize and quantify results. With the advancement in irradiators we can now use imaging to track tumors and quantify the results.
Another key topic between in vitro and in vivo studies is the value of the tumor micronenvironment. If you use cells, subcutaneous human derived tumors, orthotopic human derived tumors or spontaneous mouse derived tumors, you can create many different microenvironments. This relates directly to the blood flow, vasculature, hypoxia and growth of a tumor.
Hedgehog pathway has been implicated in tumor recurrence. Inhibitors of this pathway have shown radioresistance (hepatocellular) and radiosensitivity (esophageal) in vitro. This study on non-small cell lung cancer showed no significant radioresistance in vitro. In vivo (xenograft and orthotopic models) both showed great radiosensitivity verified by CBCT on SARRP.
To understand more about which Xstrahl system can be utilised for in vitro and in vivo treatments contact our technical team here.